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When appropriate, complex spectra were deconvoluted in order to isolate the absorbance of individual, overlapping bands.
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The deconvolution is necessary to isolate the absorbance due to [Ti] from that due to [Si] Table 4 Calculated water contents associated with each band deconvoluted from the measured spectra of [Ti] experiments.
Therefore, the CPPs eliminated the optical interference by adding a centrifugation procedure to isolate the suspended ENM at the bottom of the cell culture dish, then transferring the supernatants to a fresh plate to conduct the absorbance readings.
Then, the lipid portion was isolated and the absorbance of the drug in the supernatant was determined by HPLC at 246 nm.
Since the absorbance of irradiation at parallel polarization is obviously more than the absorbance of irradiation at perpendicular polarization in this report, which makes it challenging to compare the absorbance of vibrational bands directly.
With the MTT assay, cell survival was considered proportional to the sample absorbance, and was expressed in % to the absorbance of the sham-exposed parallel control samples.
The absorbance of the sample was compared to the absorbance of the photocatalyst sol.
The contribution of proteins to the absorbance of the mixture at 260 nm was negligible.
The results were expressed as an absorbance ratio corresponding to the absorbance of the cells treated with H2O2/untreated cells.
Results were expressed in arbitrary units as the ratio between absorbance of the test serum to 70% of the absorbance of the positive control serum multiplied by 1000.
Here, we analyzed the changes in the light-scattering properties of isolated thylakoid membranes by recording the absorbance of their suspensions in the region 400 750 nm. Figure 1A, upper panel, shows the characteristic spectra from wild type, stn7, stn8 and stn7stn8 membranes resuspended in a buffer containing 5mM MgCl2, to retain the native grana and stroma lamellae structures.
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