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The resuming of contact that suppressed the 'contactless switch' signature contributed in our culture model to increased viability of the tumor cells.
At the very least, the authors should give more information relating to the hypothesis that suppression of pectin degradation in the triploid PEG mutants contributes to increased viability.
The indistinguishable conversion rate of furfural by evolved and parental strains has been previously reported and mentioned that improved performance of evolved strain was due to increased viability under furfural stress [ 30].
Consistent with these findings were the results of in vitro studies, where downregulation of EpCAM with shRNA led to increased viability and cell growth in the SKBR3 breast cancer cell line.
In the presence of furan aldehydes though, the detoxification abilities of JWCB044 led to increased viability and biomass production when cultures were challenged with increasing concentrations of furfural or 5-HMF.
In the Materials and Methods section (" In vitro assay for BLyS bioactivity"), we have described the Alamar Blue assay which was used to assess increased cell numbers, whether due to proliferation (as in anti IgM treated cultures) or to increased viability (as in cultures containing recombinant BLyS).
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The data presented here underline the importance for C. pneumoniae to be taken up by PMN to increase viability and virulence in macrophages.
The results showed that none of the growth factors examined, including brain-derived neurotrophic factor (BDNF) was able to increase viability of NPCs compromised by IFNγ (Fig. 5A).
One such factor is the small molecule ROCK inhibitor Y27632, which has been demonstrated to increase viability of dissociated hES cells [31], [32].
Rapamycin treatment alone increased colony forming potential in cultures that did not receive IGF-I suggesting that rapamycin was able to increase viability of cells in serum free conditions although this difference was not statistically significant.
To this end, we assayed the ability of 400 compounds from the Prestwick Chemical Library (PCL), a collection of drugs selected for their biological activity, to increase viability of female flies expressing i CUG 480 RNA in their MBs.
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