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The retrobiosynthetic concept was designed to improve the stringency of isotope incorporation studies.
To improve the stringency of our analysis we considered genes as changed only if they had a t-test and a TNoM p-value <0.05 and a fold ratio >2 as previously described [20].
Schwartz et al. have proposed some guidelines to improve the stringency of the Then-test method [ 41].
In this study, in order to improve the stringency of detecting PABC and to avoid coincident cases, we restricted our sampling to tumours occurring up to 6 months after delivery.
To improve the stringency of the test, a Benjamini and Hochberg multiple testing correction was applied to the unadjusted p values with a custom R script using the multtest library [ 46, 47].
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To improve the stringency, we included only mouse database from IPA for the network/pathway analysis.
The use of the dog genome as an outgroup to human, rat, and mouse improves the stringency of the study [ 35].
Furthermore, we improved the stringency of the prediction by considering certain quantitative scores.
Increasing the level of polymorphism of the chosen loci improves the stringency of paternity analysis, by reducing the rate of multiple assignments and type II errors.
To further improve the stringency, we applied one-way ANOVA analysis on the differentially expressed genes identified by SAM.
Strategies that are commonly used to improve the method include increasing the stringency of the co-precipitation conditions and biochemical cross-linking prior to tissue solubilization.
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