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The resulting cDNA was amplified by PCR and cloned, and several clones were sequenced to identify the consequence of this mutation.
The PCR amplicons of the Agro_gc40, Agro_gc50 and Agro_gc60 sequences, of which G/C content ratios were 40, 50 and 60%, respectively, were digested using the MspJI enzyme to identify the consequence of 5mC-containing DNA cleavage in the size-resolution range afforded by a 2.5% (w/v) agarose gel.
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Through the advent of global gene expression profiling methodologies such as microarrays, it has become possible to identify the consequences of these remarkably conserved chromosomal aneuploidies on the cancer transcriptome.
This situation may help to identify the consequences of fatigue to the body, such as postural control.
Therefore, we have to identify the consequences of changes occurring in the coevolving organisms that lead to their domestication [ 1].
The Variant Effect Predictor tool from Ensembl [ 44] was used to identify the consequences of all variants in each sample.
The International Mouse Phenotyping Consortium (IMPC; www.mousephenotype.org) aims to create 20,000 knockout (KO) mouse strains over the next 10 years, with viable strains undergoing comprehensive phenotyping as adult mice in order to identify the consequences of gene disruption.
The predictive abilities of the GP stage were taken as the definitive decision criterion for identifying the best strategy for model selection, the best model, and the most reliable approach to account for year effects, and to identify the consequences of population stratification in GP.
In a problem of measuring the effect of an investment plan which including many projects, one may prefer knowing the total profit of all projects to identifying the consequence of each project.
Identify the consequences of each risk.
For instance, ANNOVAR [ 22] annotates variants relative to a number of popular gene sets to identify the functional consequence of the mutation; for example, new amino acid (missense) or stop-codon (nonsense) mutations can result from a non-synonymous point mutation.
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