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As compared to ginsenoside backbone biosynthesis, we know little about the late stages of ginsenoside biosynthesis.
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Among our results, we found all of the known enzymes involved in the ginsenoside backbone biosynthesis and used co-expression analysis to identify a number of candidate sequences involved in the latter stages ginsenoside biosynthesis pathway.
In our results, we were able to identify predicted representatives for all of the known enzymes involved in the ginsenoside backbone biosynthesis and also profile their expression levels across seasonal development.
After annotation, we discovered 223 unigenes involved in ginsenoside backbone biosynthesis.
Furthermore, our database includes all of the genes encoding enzymes involved in ginsenoside backbone biosynthesis and modification.
Expression profiling of all ginsenoside backbone biosynthesis genes suggests that ginsenoside biosynthesis is tightly linked with the developmental stages of flowering and fruit drop.
Bioinformatic analysis indicated that all genes encoding enzymes involved in the biosynthesis of the ginsenoside backbone existed in the transcriptome of the American ginseng root.
Furthermore, a few candidate genes putatively responsible for ginsenoside backbone modifications were screened out of a gene pool containing 150 CYP450 and 235 UGT unique sequences.
Based on the bioinformatic analysis described above, we found all of the known enzymes involved in ginsenoside backbone synthesis, starting from acetyl-CoA via the isoprenoid pathway.
Based on the KEGG pathway assignment, we found all of the genes encoding enzymes involved in ginsenoside backbone biosynthesis (Table 2).
Additionally, a total of 150 cytochrome P450 (CYP450) and 235 glycosyltransferase unique sequences were found in the 454 cDNA library, some of which encode enzymes responsible for the conversion of the ginsenoside backbone into the various ginsenosides.
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