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To further confirmed our assignment, we ordered standard chemicals of 3-methoxy-5-methylpehnol (CAS NO. 3209-13-0), and 4-ethyl-2-methoxyphenol (CAS NO. 2785-89-9), aNMR1H NMR spectral data of two compounds were record in DMSO-d6.
To further confirmed this idea, immunoprecipitates from Hep3B, MHCC97L and MHCC97H cells using HSP27 antibody were analyzed by SDS-PAGE, followed by western blot analysis with activated NF-κB p65, IKKα, IKKβ and IκBα-specific antibodies.
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The TUNEL assay was used to further confirm DNA fragmentation.
In order to further confirm SNP formation, TEM was performed.
Chest xray performed to further confirm the diagnosis.
However, more rescue studies are needed to further confirm it.
To further confirm this, composition EDX studies were carried out.
XPS was used to further confirm Cu loading and to probe Cu coordination sphere (Figure 2C).
2D-NMR analyses were carried out to further confirm the glucosylation position.
To further confirm this result, point or delete mutations of INO80 protein were designed (Fig. 4C).
To further confirm the formation of the samples, Raman detection was performed.
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