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noun
A narrow-necked vessel of metal or glass, used for various purposes; as of sheet metal, to carry gunpowder in; or of wrought iron, to contain quicksilver; or of glass, to heat water in, etc.
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1 g of fabric is transferred to flask containing 50 ml of the working dilution then capped flask shaken for 1 h at 250 rpm.
Changes to flask volume and cell line were observed to modify substrate carbon consumption within the cell culture, whilst having no significant impact on overall cellular yield.
In this work, we have performed a preliminary study on the effect of oxygen on the recombinant protein expression by evaluating the expression levels in shake flask cultures with different liquid to flask volume ratios.
To study the effect of OTR in SELP-1020-A SELP-1020-A SELP-1020-Ary study of productionpression with different liquid to flask volume ratios was carried out with the preliminaryoptimistudyofditions (TB+lac, 22 hours fermentation at 37°C).
For all SELP copolymers, bacterial cell cultures were grown in TB+lac containing ampicillin, with a liquid to flask volume ratio of 1 5 (200 ml of medium in 1 L flask) at 37°C.
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Culture conditions in all cases were 37°C with constant aeration and a medium-to-flask volume ratio of ≤0.50.
This removes any potential flask-to-flask differences.
The irradiation field was 40 × 40 cm and the source-to-flask distance was 80 cm giving a high dose-rate (HDR) of 30 Gy/h.
This is a rough estimate due to transition/transversion bias, among-site rate variation, and strong bottleneck effects from the flask-to-flask transfer regime.
Irradiation was performed at room temperature using a Co-60 teletherapy unit delivering approximately 1.8 Gy min−1 at 80 cm source-to-flask distance.
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