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Viral inoculum was added to small plugs of artificial diet and larvae were maintained individually and allowed to feed overnight.
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To test the effects of the MC4R selective compound AST3-88, rats were induced to feed by overnight food deprivation, a validated method to observe modulation in MC4R signaling.
The pigs were not fed overnight prior to the experiment but had free access to water.
(A ) Peak ΔF/F responses of the DM5 glomerulus to cider vinegar (left) and the appetitive index of flies (right) that were fed overnight with 4% sucrose alone or sucrose with the PI3K blocker, wortmannin.
Adult females fed on a 10% sugar solution were injected with dsRNA at 3 days post emergence and fed overnight on the same 10% sugar solution.
Mature isogenized males of the genotype y w; FRT40Aiso1 were fed overnight with 25 mM EMS in a 1.5% sucrose solution before a brief incubation in a clean vial and normal feeding.
Flies were fed overnight with 200 μl of 4% sucrose solution, or plus 25 nM wortmannin.
In Sri Lanka a significant number of children are fed overnight, beyond the age of two years [ 6].
More information could have been collected about breastfeeding patterns: data on how often women fed and the length of intervals between feeds overnight could have been useful.
In all cases of flow measurements, flies were yeast fed overnight, dissected under Voltalef 10S oil and imaged at 40× magnification in a confocal microscope.
For cold experiments, flies were kept at room temperature (22°C), yeast fed overnight at 25°C, and subsequently kept at 25°C for 6 hr before dissection and fixation.
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