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However, Cre/Lox-dependent luciferase expression was not enhanced relative to expression using hTR or hTERT promoters directly.
Taken together, this work presents an efficient strategy for expression of cspA that could be applied to other streptococcal proteins that are not amenable to expression using common Gram-negative hosts.
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Answer: Since they are equivalent to expressions using the union, dot, and star operators, there is certainly some way to do the translation to an epsilon-NFA.
(c) FACS analysis of CD34+CD61+ and CD34+CD61- sorted cells after 5 days in ECM culture to CD61 expression using gates according to sort experiments.
All reactions were performed in triplicate on each plate with three independent replicates, and gene expression values were calculated using the difference in eng expression relative to act1 expression using the method 2−ΔΔCt (Livak and Schmittgen 2001).
The expression of each gene was normalized to GAPDH expression using the following formula: 2- gene CT value – GAPDH CT value).
Expression levels of targets analyzed were calculated relative to GAPDH expression, using the ΔΔ Ct method.
Fold-change expression of 'test' mRNA was determined relative to RPL32 expression using the 2−ΔΔCt method.
We used the "Detection Score" to determine expression using the Illumina platform.
The conetwork phase diagram is very sensitive to the expression used to describe the energy for loop formation.
To confirm this conclusion, we sought to knockdown PP2A expression using RNA interference (Fig. 4).
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