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To exclude potential errors from body weight variations, liver/body weight ratios were used to assess remnant liver restoration.
To exclude potential errors in measurements of low protein concentration, we stably expressed H2B-RFP in CAY/− cells and determined that 0.73% of nuclear CENP-A is present on each centromere.
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The computation was then manually validated to exclude possible errors.
This allows to exclude potential biasing effects of infant mortality.
We also sequenced the genome of BN/Mcwi, the rat strain from which the reference BN rat genome was derived, on the SOLiD sequencing platform, using protocols previously described (Simonis et al., 2012 ), to identify and exclude potential sequencing errors in the reference genome.
After each step, the data were visually inspected to exclude processing errors.
Samples were re-analyzed to exclude technical errors.
42 All electrocardiograms were reviewed to exclude coding errors due to technical causes.
To exclude systematic errors due to grid artefacts, all computations were performed on different grids.
In case of discordant results, both tests were repeated to exclude manipulation errors.
A careful reinspection of such a data is always needed to exclude sequencing errors.
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