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Staining for cardiomyocyte markers was used to evaluate the alignment and spreading of cardiomyocytes.
Two parameters, contiguity and completeness as described by Martin and Wang [ 85] were used to evaluate the alignment results.
Polarizing light microscopy was used to evaluate the alignment of collagen fibers in Picrosirius red-stained sections.
In cases where small RNAs had an alignment length of less than 16 nt, we used the Smith-Waterman score in FASTA v36 (http://faculty.virginia.edu/wrpearson/fasta/fasta36/) to evaluate the alignment.
The second approach is to evaluate the alignment for specific features difficult to take into account while building the MSA, but measurable on a complete model (e.g. completely conserved positions) (Thompson et al., 2003).
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Another author, MM, carried out the automatic word alignments in 6 batches with different configurations to be able to evaluate the alignments.
To evaluate the alignments, they are mapped to the reference alignment using an intermediary sequence; for BLASTN, this is the nearest neighbor sequence it was aligned to, and for the profile methods it is the consensus sequence of the model.
Therefore, there are eight (λ, K) parameters that help to evaluate the alignments (four for the forward alignment sense and four for the reverse complementary alignment sense): (λ FW, K FW ) for the forward sense and (λ RC, K RC ) for the reverse complementary sense respectively, that are used for evaluating the score of the whole alignment.
This tracing algorithm is freely available to the scientific community under the name Neurient, and its implementation in Matlab allows a wide range of researchers to use a standardized, open source method to quantitatively evaluate the alignment of dense neuronal cultures.
In order to facilitate this work we propose a simple template to be positioned on the radiographs, which will make it possible to rapidly evaluate the alignment of the metatarsals and the corrections necessary for re-establishing correct architecture of the forefoot.
PORTAAL, like any tool, was designed with a particular purpose and scope: to reliably evaluate the alignment between instructor implementations of active learning and research-supported best practices in the classroom.
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CEO of Professional Science Editing for Scientists @ prosciediting.com