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Treatment of wild-type ESCs undergoing the same differentiation protocol with 4-OHT did not affect their ability to differentiate (supplementary material Fig. S1C).
Surprisingly, a transient ectopic re-expression of Copr5 in KO cells failed to rescue their capacity to differentiate (supplementary material Fig. S2C).
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Under neurosphere differentiation conditions, all the GSK-3 knockout neurospheres, except the DKO, are able to differentiate into neurons (supplementary material Fig. S2C).
Furthermore, the established BM-MSCs had the capacity to differentiate into adipocytes and osteoblasts (Supplementary Fig. 1b).
Measurements of rCBV (DSCE-MRI) can also provide supplementary information to differentiate between malignant lymphoma and glioma because the absence of tumour neovascularization in malignant lymphoma leads to low rCBV in contrast to those of malignant gliomas[ 61 ].
However, N-Myc is likely to be a critical Wig-1 target for induction of differentiation as the SH-SY5Y neuroblastoma cells that do not express N-Myc fail to differentiate upon Wig-1 knockdown (Supplementary Figure S3b and c).
This partial redistribution of L91P and A212P seipin was observed in all cells expressing these constructs whether subconfluent pre-adipocytes (Fig. 2e) or post-confluent cells induced to differentiate for 48 h (electronic supplementary material [ESM] Fig. 1).
We analyzed apoptosis in PSN-1 cells by Annexin V-FITC/PI to differentiate necrotic from early apoptotic cells (Supplementary Figure S4).
The more rapidly evolving intron sequences, however, allowed us to differentiate between Y- and X-linked scaffolds (supplementary fig. S1, Supplementary Material online).
Cells of all passages were able to differentiate in vitro with close to 100% differentiation efficiency (Supplementary information, Figure S3F and S3G), suggesting that the differentiation potentials of MuSCs remained intact after serial expansions.
The captured cells retained the ability to differentiate in vitro into all three germ layers (Supplementary information, Figure S3c).
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