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(A) Sanger sequencing to detect the genotype of the patient.
Thus, we designed a simple PCR fragment length assay to detect the genotype of indels in APL1A (Figure 4B, C).
Di-allele -specific-amplification with artificially modified primer (di-ASA-AMP) was used to detect the genotype of CYP1B1 C1294G [ 18].
Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was performed to detect the genotype of the single-nucleotide polymorphism (SNP) rs708567 in the IL-17RC gene.
We failed to detect the genotype of few patients, both in rs12917716 and rs6498169, and these patients were excluded from the statistical analysis.
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STS10-16 and STS10-27 were used to detect the genotypes of plands in the F2 populations derived from the NIPA/L30 and NIPA/L47 crosses, respectively.
RM407 was subsequently used to detect the genotypes of plants in the F2 populations derived from the following crosses: NIPA/L1, NIPA/L18, NIPA/L19, and NIPA/L20.
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes of rs4379368, rs10504861 and rs10915437, and direct sequencing was used to evaluate rs12134493 and rs13208321.
Our recommended strategy is (1) to conduct whole-genome sequencing of several representative cultivars in a set of accessions (selected from ecotypes identified by population structure analysis or from genetically similar breeding lines) and (2) to detect the genotypes of all the lines in the population by using SNP typing arrays.
Depending on the platform used to detect the genotyping products, the benefits of this four-primer, biphasic PCR mechanism range from increased sensitivity and specificity for allele-specific primer extension chemistry to size-controlled PCR products for high resolution melt detection.
This suggests that the mothers can detect the genotype of pups.
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