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Western-blot and immunofluorescence assay were processed to detect the expressions of AIF, Cyt C, Bax, Bcl-2 in brain.
In addition, IHC probes in specimens from the two patients were applied to detect the expressions of CD99 (DakoCytomation, Carpinteria, CA) and nestin (abcam, Cambridge, MA).
DIG- and FITC-labeled riboprobes were used to detect the expressions of 2 genes.
Immunoblotting was used to detect the expressions of EGFR Ras Raf MEK ERK signaling pathway proteins.
In addition, we performed western blotting to detect the expressions of cleaved caspase-3 and PARP.
WB method was adopted to detect the expressions of CRH and GR protein in PVN.
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Semi-quantitative PCR was used first to detect the expression of testis/ovary specifically expressed genes.
However, the results were not satisfactory, since we were not able to detect the expression of several constructs, and some others were expressed in aggregated form.
Northern blots were further performed to detect the expression of miRNAs.
Next, immunohistochemistry was performed to detect the expression of COX-2 protein in 88 prostate cancer tissue samples.
In this case, a FITC-ICAM-1 antibody was used as an indicator to detect the expression of ICAM-1 on the surface of Raw264.7 cells.
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