Semi-quantitative PCR was used first to detect the expression of testis/ovary specifically expressed genes.
Northern blots were further performed to detect the expression of miRNAs.
Next, immunohistochemistry was performed to detect the expression of COX-2 protein in 88 prostate cancer tissue samples.
Eventually, supernatants were collected to detect the expression of IFN-γ and IL-17.
Noticeably, we were not able to detect the expression of LC3A mRNA in cytotrophoblastic cells.
We had developed a set of PCR assays to detect the expression of each v-rDNA.
Thus, immunohistochemistry was used to detect the expression of IRF-1, p21 and BAK.
Western blotting was performed using a goat anti-V5 antibody (Abcam, Cambridge, MA) to detect the expression of all V5-tagged candidate genes, and a goad anti-GAPDH antibody (Abcam) to detect the expression of GAPDH as a loading control.
Recently, several instruments have been described that provide alternative formats to detect the expression of multiplexed genes in small samples.
To detect the expression of pre-miRNAs, total RNA was extracted from MEF using the RNeasy mini kit.
Standard western analysis method was carried out to detect the expression of G6 in transgenic rice plants.
