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The time duration to define the background level (N in Fig. 1) was from 0.02 to 8.192 ms.
The effect of the semantic layout algorithms is illustrated in Fig. 4b, where the same property used to define the background color is also used as basis for the similarity calculation.
Naked genomic DNA randomly digested with micrococcal nuclease was also hybridized to tiling arrays and used to define the background.
The non-invasive S. carnosus strain TM300 (ATCC 51365) was used as negative control to define the background of the fluorescence signal.
We then tested PHD2 interaction with GRP94 to define the background level of fluorescent emissions of the acceptor protein EYFP in our FRET system.
Serum samples of naïve mice were used to define the background of the gD2-antibody capture assay, and the abundance of gD2-antibody in all serum samples was normalized to this background value.
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Unmethylated commercial DNA (Qiagen, Valencia, CA, USA) and mixed human lymphocyte DNA (Promega, Madison, WI, USA) was analyzed in parallel to define the technical background.
To this end, the standardized assay was used in series of experiments with more than 20 donors to define the expected background spot production and intra- and inter-assay variation in the hands of defined operators.
Both of these phenomena are used as biomarkers for estrogenic effects and as always in the use of biomarkers, it is important to define the natural background level in an uncontaminated environment.
The color segmentation method needs the user to define the grain and background colors.
The average intensity of 8,421 random probes was used to define the global array background.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com