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The greatest degree of inhibition was observed in pre-treated target cells, but modest inhibition of infection was also seen when drug was added to cultures up to 48 h after infection.
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Although deletion of acdX did not lead to derepression of alcA in liquid cultures, up to 12-fold elevation was detected in induced conditions.
But what happens to the people who are trying to push culture up from the ground floor?
Cell cultures up to the fifth passage were investigated to confirm purity using specific fibroblast antibodies.
To interrogate this we prepared and cocultured MDM with HIV-1+ T cells, washed and maintained cultures up to 72 hr ± AZT, and measured vDNA content.
All previous and all sequential stool cultures up to day 14 of this subject were negative.
Bacterial inocula were prepared in 0.9% sodium chloride from fresh cultures up to the moment of 0.5 McFarland's turbidity.
"We're looking to bring culture up, not down".
We need to get culture up on that same international platform".
Of course, it's hard to open our culture up to the judgment of others without feeling vulnerable.
This protocol should provide continuous exposure to PTH because PTH has been shown to be stable in culture up to 72 h between medium changes [ 35].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com