The mutation is predicted to create a premature termination codon at position 757 (p.Glu757x).
Auto-Scale Engine analyzes resource information stored in Resource Information Manager, decides the creation or termination of virtual machines according to the load of ACCP, and asks Resource Provider to create or terminate virtual machine instances.
The rationale for mild termination is as follows: Termination must be strong enough to create 5' overhangs in the first PCR reaction, but weak enough to allow the polymerase to continue through the modified nucleotide during the second round of PCR (Fig 2).
Open reading frame (ORF) analysis predicts that omission of the 187-nt exon 3 introduces an in-frame premature termination codon, UAA in exon 4. Introduction of a premature termination codon in the exon 4 is predicted to create a short ORF, encoding a C-terminal truncated peptide of 80 amino acids (Figure S5, see ORF1).
The other way of doing that would be to look at sanctions terminations as an insurance policy, to create a different type of interaction with Iran.
But "given the magnitude of further cost reductions needed to create a viable business plan and attract exit financing, termination and replacement of all our defined-benefit pension plans likely will be required," United said.
For circular chromosomes, the origin of replication may be used in place of the centromere, while the location of termination can define a cutting point to create short and long arms.
To determine whether the B region mutations were responsible for the termination phenotype, we used site-directed mutagenesis to create rpb2 alleles containing the individual changes.
The primers used were designed to create an NdeI and a BamHI sites at the initiation and termination codons, respectively.
Programmed fork pausing and replication termination events near the mating-type (mat1) locus are needed to create an imprint and initiate a gene conversion event that switches mating-type in fission yeast [48], [49].
