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When they started the project, they didn't just want to create some variant of JavaScript.
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For example, Afriat-Jurnou et al. have remodeled Loop 7 of PTE to create a variant of the enzyme that led to the emergence of homoserine lactonase activity that is undetectable in wild-type PTE.
In the ruins, new small group may undertake an attempt to create another variant of the species, which may happen to be more perfect than its predecessor and live longer.
To address this problem, Wong has used directed evolution to create a variant of l-rhamulose-1-phosphate aldolase (RhaD) which accepts dihydroxyacetone (DHA, 8) as the donor (Scheme 2) [49].
To create siRNA-resistant variant of the genes, the following sequences were mutated using the Quikchange II site directed mutagenesis kit (Stratagene) (underlined are the silent nucleotide substitutions).
To create a Q698E variant of Hsp90rr, pUC Hsp90rr was amplified using the phosphorylated primers Hsp90.Q698E.fwd (AGCATGGAGG AGGTGGACTG AGGATCCTCTAG) and Hsp90.Q698E.rev (GGAGGTGCCG GCGGTGACC).
Thirty clips were selected to create two variants of the hazard perception test: a traditional push-button test requiring speeded-responses to hazards, and a prediction test that occludes at hazard onset and provides four possible outcomes for participants to choose between.
A random noise varying from 1 to 10% (with the step of 1%) was added to both original MSAs (for the Primate and PheRS data) to create the variants of "noisy" data.
For the first experiment we develop synthetic voices of school children, their teachers and of speakers that are unfamiliar to them and use each of these voices to create variants of two audio games: a memory game and a labyrinth game.
The group of unpaid volunteers plans to create four different variants of the baker's yeast to make the cheeses.
Therefore, to create variants of the HscA NBD containing a partial or full interdomain linker, we utilized structures of ADP- and ATP-bound DnaK (PDB entries 2kho and 4jne, respectively) and a bioinformatics approach to identify conserved linker residues among various Hsp70s.
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