Quantitative Real-Time PCR experiments were carried out to corroborate the expression patterns of a subset of sequences (i. e. 89 out of 642 unisequences, equal to 14%), corresponding to 61 different genes.
To corroborate the simultaneous expression of lymphatic markers and GFP in individual cells, single cell suspensions from tumors of GFP+ bone marrow-transplanted or control non-transplanted mice were analysed by FACS (Figure 2B).
Addition of 500 nM tetrodotoxin (TTX) to the extracellular solution abolished the inward current, corroborating the expression of TTX-sensitive sodium channels, INa, in these cells.
We corroborated the expression of miR-200c and miR-141 in the same set of normal mammary samples by real-time PCR, and then expanded these results to pairs of epithelial cells and fibroblasts from prostate and skin, as well (Figure 1B; Figure S1).
We corroborated the expression of each candidate gene in microarray analysis by quantitative real time-RTPCR (qRT-RTPCR).
A - Double FISH of Smed-nf-YA, Smed-nf-YB-2, and Smed-nf-YC shows colocalization of the NF-Y subunits with the neoblast marker Smed-h2b, corroborating the expression of this complex in neoblasts.
Further bioinformatic analysis of the data to examine cell markers, and genes whose expression may correlate with them, in combination with analysis of the cell populations present in the arthritic joint using immunohistochemistry or fluorescence activated cell sorting techniques, would be required to corroborate the differential gene expression of a particular gene of interest.
We make specific and anecdotal observations for each compartment below to corroborate the role of periodic expression in each.
To corroborate the validity of miRNAs expression of FFPE samples, three miRNAs were detected in 10 matched FFPE and snap-frozen samples from the same patients.
To corroborate the findings of differential gene expression, we used genetic and pharmacological approaches to activate or inhibit ERα, ERβ1, or ERβ2 selectively in 3 4 dpf transgenic zebrafish.
