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After this characterization of the communities, half of the communities were translocated between the two sites within a region while the other half was back-transferred to their site of origin to control for transfer effects without site change.
A pre-stained protein ladder (PageRuler Plus, Fermentas Life Science) was loaded into the gel to control for transfer efficiency.
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With a reduced number of sequences, one can more accurately remove ambiguously aligned positions in each file, and construct preliminary phylogenetic trees of each gene to control for laterally transferred or paralogous genes (Fig. 3, step 4).
All membranes were blotted with an anti-Hsc70 (mouse monoclonal, Santa Cruz Biotechnology) antibody to control for loading and transfer.
In order to control for effects of the transfer process per se, I also carried out transfers of neonates obtained from crosses with mothers of one Mstn genotype to foster mothers of the same Mstn genotype.
α-tubulin antibody was used to control for equal loading and transfer of the samples, as mentioned previously.
Experiments involving immunoblotting procedures were repeated at least three times and blots were re-probed with an antibody for β-actin to control for protein loading and transfer.
Membranes were blocked with 5% milk and then incubated with α-tubulin antibody (B-7 sc-5286, mouse monoclonal 1∶600, Santa Cruz Biotechnology, CA) to control for equal loading and transfer of the samples.
Alpha-Tubulin antibody was used to control for equal loading and transfer of the samples.
Blots were re-probed with an antibody for β-actin to control for protein loading and transfer (data not shown).
Blots were re-probed with an antibody for β-actin to control for protein loading and transfer.
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