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However, it took from 4 to 20 years to confidently detect the effects.
This required detectors of unprecedented sensitivity; to confidently detect the tiny signal of a broken nucleus, all other extraneous radioactivity has to be reduced to the lowest possible level, and measured precisely.
Although the 1000 Genomes Project (1KGP) has offered brief insights into the value of population-level sequencing, the low coverage has compromised the ability to confidently detect rare and low-frequency variants.
The results of this study showed that the proposed approach was able, in a rigorous statistical framework, to confidently detect the presence of structural changes, accurately locate the structural section where the change occurred, and provide an accurate estimate of the actual level of "change".
Using standard parameters to test at the 5% significance level and with a 90% power, the minimum population size required to confidently detect small hazard ratios would be 516, thus it was safe to assume that the sample size was large enough to produce statistical significance.
The trials were small and underpowered to confidently detect or exclude effects.
Similar(33)
A few loci are in fact expected to have high enough effects to be confidently detected.
We considered a COG to be confidently detected in a sample if its relative abundance exceeded 10−4.
Due to the fact that air pollution data was only available for a shorter time period, we couldn't confidently detect a seasonal pattern within this data (see Additional file 1, Figure S2).
The standard DR*W201/P65 tetramer staining method could not confidently detect vaccine-elicited P65-specific memory CD4 T cells in PBMC of the BCG-vaccinated Mamu-DRB*W201+ macaques, because 0.03∼0.04% frequencies were too low to distinguish them from background staining (left bar graph of 5a).
A notable observation in this construction was that by using highly selective PCR (with one of the primers annealing within gfp), we could confidently detect the mutant in every one of 40 plaques examined, reflecting a remarkably high level of mutagenesis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com