Moreover, while the exogenous expression of an RNAi-resistant full length MCPH1-transcript was sufficient to rescue this effect of the MCPH1 knockdown, a transcript that was isolated in a human breast cancer specimen lacking the C-terminal BRCT-domains was not able to complement the defect.
However, the RecA-GFP protein was unable to complement the defect in SOS induction.
However, the N-terminal extension (aa 1-50) alone is not able to complement the defect (data not shown).
pUL34 R153despiteite showing a nuclear envelope staining, could not recruit WT pUL31 to the nuclear envelope and was also unable to complement the defect of the deletion mutant.
However, the ability of RecBCEc enzyme (lacking RecD), unlike the RecBCPs, to complement the defects of LCBD strain was a little surprising.
The ability of the RecA-GFP chimera to complement the defects of the Δ recA strain in growth, viability, SOS induction, UV sensibility, genetic recombination and swarming motility was compared to that of the RecA-Htg variant.
For instance, the presence of one allele expressing the p.Ala428Thr variant, which, in yeast, is of intermediate severity, is probably not sufficient to complement the defects caused by the variant Arg620Lysfs*8, which is functionally null.
As a result, the fzy-1(h1983) mutation partially complemented this defect and suppressed the phenotype of bub-1 fw8). bub-1 fw8
Truncation of the G-patch domain of Pfa1 is unable to complement growth defect of cells lacking Pfa1 and Ltv1.
Expression of the wild-type gene does not provide any survival advantage to the transduced stem and progenitor cells thereby necessitating the use of myeloablative conditioning to complement for this defect.
