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We isolated three new recessive par-2 mutants that disrupt zygote polarity; all three alleles failed to complement the previously identified allele par-2 lw32) par-2 lw32shown).
We found one new lit-1 mutant, or393 ts and one new mex-1 mutant, or286 ts, which failed to complement the previously identified alleles lit-1 or131 lit-1 or131-1(zu120), respectsvely (dand mex-1 zu120 mex-1 zu120
We isolated four new recessive alleles of spd-2 that cause a variety of centrosomal and microtubule-related defects, and all four alleles failed to complement the previously identified allele spd-2 or188 spd-2 or188ts not shown).
Similar phenotypes have been described previously for other alleles [47], and both mei-1 or642 mei-1 or642-1(or646 ts) fanded mei-1 or646 mei-1 or646ously identsfailedllele mei-1(b284) (dato not shown).
The enhancer allele dz147 behaved recessively, showed linkage to chromosome V, and failed to complement the previously identified enhancer ot21.
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Data collected through the individual interviews served to triangulate and to complement the information previously gathered through the documentary review, while information from the documentary review served to further explore regional particularities during the qualitative interviews.
To complement the in vivo data previously obtained with cells isolated from primary spheres (ex Figure 3 figure supplement 5), we transplanted secondary spheres harvested from untreated and HGF-stimulated cultures of luminal progenitors.
To the best of our knowledge, there are very few works concerning the studied problem, which implies that the results of this paper are more general, and they effectually complement the previously known results.
These results suggest that PSE has the capacity to complement the markers that we have previously identified.
Of these homologs, the least diverged ortholog, h CDC42 (80% sequence identity, previously shown to complement the yeast deletion mutant (Kachroo et al. 2015), was not available in the human ORFeome V8.1 collection and therefore not tested in this study.
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