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Although norms would suggest a hypothetical best operational workflow as an outcome of this paper, we instead argue that the identification of circumstances in which different methods are combined to complement the limitations of another is more beneficial.
However, other fields are needed to complement the limitations of the genomics approach.
In the meantime, animal experiments and clinical intervention trials with validated surrogate outcomes of CVD will be essential to complement the limitations of the evidence from observational studies.
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2) Phylogenetic analysis (Figure S4) in combination with tree reconciliation, which is useful to complement the limitation of the "reciprocal best hits" method.
To complement the limitation of the high k-mer that we selected for the Velvet assembly in this study (lower diversity and probably biased toward highly expressed genes), we generated additional ESTs using the GS FLX Titanium platform.
We propose an experimental pipeline that uses the Illumina assembled contigs, in addition to just the reads, to complement the current limitations of the PacBio sequencing technology and assembly software.
From their experience, GPs expressed a need to rely on their 'common sense' a source of 'knowledge' that may complement the limitations of guideline application in multimorbidity.
Furthermore, the MDV database provided objective information that would not only complement the limitations of the claims database, but that would also provide the potential to monitor pharmacological responses to therapeutic interventions using biomarkers.
Thus, organ culture of the vasculature has been used as an alternative method that can complement the limitations of animal- or cultured cell-based studies for investigating the chronic treatment effects of various inhibitors in arterial tissues (Ozaki and Karaki 2002 Thorne and Paul 2003 Huh et al. 2011).
Importantly, GFP::SBP/ spl7-2 lines were not only able to complement the spl7 mutant phenotypes under Cu limitation with respect to root growth, size or silique production (Additional file 4: Figure S4), but a GFP signal also became microscopically detectable in planta.
To redress this limitation, qualitative methods have more recently been used to complement the results obtained by quantitative studies.
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