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The first cycle at which the fluorescence passes from background levels to clearly detectable is called the threshold cycle (Ct).
Incubating INS-1 β-cells with 21 μM hIAPP led to clearly detectable toxicity; cell viability was reduced to 62±4%4% relative to that of the medium alone control after incubation for 24 h and to 54 ± 5% after incubation for 48 h.
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The Nup98 gel is thus more restrictive than authentic NPCs, which allow GFP-sized molecules to pass at clearly detectable rates (Mohr et al, 2009).
We chose to allocate a presence score to any clearly detectable band.
As regards CYP2E, our results showed that the intestinal gene expression detected in the PCRs did not correlate to any clearly detectable CYP2E protein expression in the western blots.
We here assume a pre-seismic strain signal to be clearly detectable if it reaches a minimal value of twice the standard deviation.
On the fluorescence microscope, for each image the CD31 fluorescence was set to a clearly detectable level by individually adjusting the gain of the CCD camera before the images were captured.
Analyses depended upon a threshold cycle (Ct) that corresponded to the first clearly detectable increase in fluorescence secondary to SYBR green incorporation into double-stranded DNA.
Therefore, to produce a clearly detectable whole-cell current, activation of individual α7* nAChRs has to be well-synchronized which may be difficult to achieve in whole-cell experiments in brain slices.
TRAF6 did not bind IRF-3, whereas binding of TRAF6 to IRF7 was clearly detectable (Figure 5A), as previously reported [11] [13].
By contrast, in the presence of PNU-120596, 10 µM choline applied to ACSF elicited clearly detectable responses (Figures 4B and 4D).
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