We believe that a better way to explore the real mutation pattern is to directly capture mutations during bacterial replication.
Our approach is shown to sensitively capture mutations that change pathway level mRNA expression, concurrently discovering important mutations previously reported in breast cancer such as TP53, PIK3CA, and RB1.
An advantage of this approach for antigen generation is its ability to capture HIV mutations which evolve under dynamic host CTL pressure [11].
The forward mutation frequency for a number of mutagens has been determined in C. elegans using the reciprocal translocation eT1 to capture lethal mutations (Rosenbluth and Baillie 1981; Adames et al. 1998; McKim et al. 1988 , 1993 Johnsen and Baillie 1988; Rosenbluth et al. 1985; Stewart et al. 1991).
This suggests that the sample size and number of microsatellties from Data set I may be sufficient to capture the mutation rate variation of the major class of microsatellites at the genomic level.
With RNAMute [ 41] it is possible to capture this mutation inside a list of about 60 other selected single point mutations that can potentially induce a conformational rearrangement, all resulting in a common or very similar secondary structure when checked with mfold or Vienna's RNAfold.
Herein, we use a pentanucleotide (which includes two nucleotides upstream and downstream of the mutation) as the basic motif and a 32 × 12 somatic autosomal mutation matrix (SAMM) to capture the overall mutation pattern within various types of cancer.
In addition, haplotypes can better capture mutations in more than one loci.
Although this project can generate more mutations in shorter time, their method does not capture mutations that exhibit lethal phenotypes, and thus, essential genes are selected against.
In their paper " Pathway-driven discovery of rare mutational impact on cancer," T. Ahn and T. Park suggested a new approach for discovering rare mutations that have real impact in the context of pathway, which could sensitively capture mutations that change pathway level of mRNA expression.
