Rats were allowed to recover for 3 days prior to behavior testing.
Where this was a potential issue (i.e., presence of descending axons in the line) we circumvented the problem by ageing the animals for 24 hr after the decapitation to allow the severed axons to degenerate prior to behavior testing, or by blocking thoracic expression using tsh-GAL80 and showing that the behavioral response disappeared.
(F ) Log-likelihood of the predictions of the stimulus-to-behavior test GLM compared to the controls.
Before starting the social interaction test, animals were habituated twice (once per day, 2 d before the day of testing) to the behavior test room for 15 minutes and then to the social interaction arena for 5 minutes on each occasion.
Shelters are wrestling with whether to abandon behavior testing altogether in their work to match dogs with adopters and determine which may be too dangerous to be released.
The offspring (vitamin A depletion group: male n = 14, female n = 14; control group: male n = 14, female n = 14) were transferred to the behavior testing facilities at 9 weeks of age and allowed 3-8 days to acclimatize before behavioral testing.
Although these mouse models display a range of psychiatric disease-related behavioral phenotypes, many have been inconsistent across studies due to differences in behavior tests and experimental design and the specific genetic aberrations.
Mice were acclimated to the behavior testing room for 1 h before ECoG recordings.
A subset of animals was used in Experiment 2b and treated sequentially with EB (0, 2.5, 5, or 10 μg) followed by TP (0, 200, or 400 μg, in a Latin-Square design) 48 h prior to sexual behavior testing.
On days when VPA or the vehicle was administered (i.e., days 0 to 6), behavior testing was performed before drug injection.
Ten male rats from each group were subjected to the sexual behavior test.
