Sentence examples for to be maintained overnight from inspiring English sources

Insulin delivery was gradually reestablished by closed-loop therapy 1 h after the hypoglycemic episode was treated, allowing euglycemia to be maintained overnight.

These conditions were maintained overnight to allow the fish to acclimate.

MCF-7 cells (150 000 cells) were added to each well and plates were maintained overnight in DMEM supplemented with 10% FBS, penicillin (20 units ml−1) and streptomycin (20  μg ml−1), at 37°C, to allow the cells to adhere to the plates.

IPTG was added to 0.5 mM and protein expression was maintained overnight at 20°C.

Prior to bloodfeeding, the mosquitoes (4 6 days post-eclosion) were maintained overnight on water only.

Cells were maintained overnight and the next day, switched to a differentiation medium consisting of growth medium with reduced FBS (2% final) and 8 µM retinoic acid (Sigma, St Louis, MO).

The plates were maintained overnight at room temperature on a shaker to dissolve the dye.

All cultures were maintained overnight then Alamar Blue (Biosource International) was added to a final concentration of 10% (v/v).

For assays done on cells at steady-state, cells were maintained overnight in a media containing 2% or 0.2% FBS prior to lysis.

HCMEC/D3 cells in culture flasks, chamber slides and transwell setup were maintained overnight in media containing 1% FBS with no growth factors (referred to as low serum media).

Before drug treatment, the medium was switched to Dulbecco's modified Eagle's medium supplemented with 2% fetal bovine serum and the cultures were maintained overnight.