Furthermore, porcine sialoadhesin-specific albumin immunoconjugates were shown to be internalized in macrophages and immunization with these immunoconjugates resulted in a rapid and robust induction of albumin-specific antibodies, this compared to immunization with albumin alone.
Approx. 6.7 logCFU was found to be internalized in case of S. Typhimurium isolate and approx. 7.4 logCFU was internalized in case of S. Typhi isolate (Table 4).
Be it as it may, it follows that norms do not need to be internalized in order to affect action.
Two classes of models have been proposed to explain why ligand needs to be internalized in order to signal [2] [7].
We tested by confocal microscopy the ability of anti-uPAR R3 antibody to be internalized in HEK293-uPAR cells transfected with the Eps15 mutant.
In support of the latter option, LFA-1 was found to be internalized in CHO cells and neutrophils (Fabbri et al., 2005; Fabbri et al., 1999).
It was observed that engineered nanoparticles can cross the plasma membrane to be internalized in cells where they may cause apoptosis [ 18] and interact with proteins [ 19].
Palmitoylation regulates NCX1 inactivation, possibly influencing the ability of the XIP region to inhibit exchanger activity, and palmitoylation promotes the participation of NCX1 in membrane domains that can be internalized in a cargo-dependent fashion.
As negative control of the procedure we used p75NTR [the neurotrophin receptor known to be internalized via a clathrin-independent pathway in neurons [70]], that indeed does not coimmunoprecipitate with clathrin.
As expected m67 potently inhibited binding of IGF2 to cells expressing the IGF1R and its phosphorylation, and resulted in formation of multimolecular complexes when incubated with IGF2 and bound with high avidity to cells expressing FcγRII; the complexes were internalized in a macrophage-like cell line.
