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Extreme care was taken to avoid variation in total biomass of the larvae in each replicated tanks and also for the entire experimental sets.
To avoid variation in DNA recovery, no further purification was performed prior to qPCR.
To avoid variation from responder T cells, we purified responder CD4+ T cells from one single healthy donor.
Average (over all loci) correlograms are presented to avoid variation in correlogram profiles based on the frequencies of individual alleles that are subject to stochastic processes.
A standard DNA extraction protocol was used for all samples to avoid variation in composition across sample type due to extraction method.
All the tasks were carry out in end expiratory, open glottis apnea in order to avoid variation in autonomic activity due to breathing.
To avoid variation due to circadian rhythm, blood was drawn in the morning between 8 and 10 am after a period of overnight fasting.
We excluded studies that assess diet using stomach content or culled prey remains to avoid variation caused by different sampling methods and studies where a large portion of the diet consisted of material other than insects (e.g., fruit, vertebrates, arthropods).
Special care was taken to avoid variation in the dose given.
These are highly dynamic processes, and to avoid variation, we took samples at consistent times.
Test samples and reference samples were dye-swapped to avoid variation arising from the dyes.
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