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Among them were the financial and fintech houses that were eager to avoid SEC scrutiny of their cryptocurrency holdings but were happy to use blockchain technology to speed up transaction times.
The 17 defendants are charged with breaking nearly every rule in the book touting stocks that they were simultaneously selling themselves; bribing brokers to push these stocks; acting as a broker-dealer without a license; and setting up shell companies off-shore to avoid SEC registration of stock in U.S. companies.
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Since out-competing control cells were not informative to test the initial hypothesis, we screened the phenotypic outcome at 3 dpf to avoid losing Sec 13 deficient clones (Fig. 6D H).
The direction of rotation reversed every 1.5 sec to avoid adaptation.
Synthetic lyophilized Aβ40 (Bachem, Bubendorf, Switzerland) was dissolved in dimethylsulfoxide (DMSO) to a concentration of 0.4 mg/ml and sonicated in ice water for 20 sec to avoid pre-aggregation.
Then the specimens were immersed in 95% ethanol for 2 3 sec to avoid contamination, and washed with phosphate-buffered saline (PBS) and antibiotics several times to remove the blood.
Synthetic lyophilized Aβ40 or Aβ42 (Bachem, Bubendorf, Switzerland) was dissolved in dimethylsulfoxide (DMSO) to a concentration of 0.4 mg/ml and sonicated in ice water for 20 sec to avoid pre-aggregation.
The cut-off time was set at 20 sec to avoid tissue damage.
Cut-off reaction time was 10 sec to avoid tissue injury during the process.
The cut-off reaction time was fixed at 10 sec to avoid tissue damage.
One microliter of cDNA was used in a PCR reaction with the following cycle conditions: 94°C 30 sec, 55°C 30 sec, 72°C 30 sec for 30 cycles (to avoid the PCR plateau).
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CEO of Professional Science Editing for Scientists @ prosciediting.com