Deparaffination/rehydration was carried out by two changes of 5 in: Xylene, 100% - 96% - 70% ethanol, TBS (50 mM Tris HCl pH 7.6, 150 mM NaCl) including drying of the membrane between xylene and 100% ethanol and between 70% ethanol and TBS which avoids detaching of the membrane from the glas.
Moving diagonally avoids detaching recently laid grout from the grout joints.
Nevertheless, documenting the process and success of scale-up is itself important if development initiatives are to foster participation and remain accountable to communities, and to avoid cumbersome organisational structures that are detached from their grassroots bases [ 23].
However, to avoid membrane stress, adherent cells were detached before Annexin V-FITC binding with accutase/low EDTA instead of the standard trypsin/EDTA detachment, since trypsinization or scrapping of the cells was shown to lead to increase of Annexin V binding (PS exposure) (PhD thesis Maximilian Martin Gemeinhardt, Munich, Faculty of Medicine, 2008).
Federal prosecutors charge that Blackwater shipped the weapons overseas with the barrels detached in an effort to avoid export regulations.
Four gunners were washed overboard at 16:56 as the ship made a sharp turn to avoid dive-bomber attacks; a destroyer was detached to rescue them, but they could not be found.
Westerners are more "analytic" in their thinking, tending to detach objects from their context, to avoid contradictions and to rely more heavily on formal logic.
Care was taken to avoid injury to the interosseous artery, vein, and nerves when detaching the flexors from the interosseous membrane.
The author argues for detaching normative objectives from 'global health' definitions to avoid so called 'entanglement-problems'. Instead, it is argued that the proposed concept constitutes an un-euphemistical approach to describe the inherently politicised field of 'global health'.
