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A window is used in the Laplace domain to avoid amplification of the Gibbs oscillations that are caused by the truncation of the spectrum.
To avoid amplification of nuclear DNA the primers were designed to amplify across exon-exon boundaries.
To avoid amplification of undesirable genomic DNA, we designed primer pairs flanking introns.
This was to avoid amplification of the short overlapping fragments during the multiplex step.
To avoid amplification of genomic DNA, samples underwent DNAase treatment using the Qiagen RNase-free DNase kit.
To avoid amplification of contaminating genomic DNA, both primers from each set were specific to different exons, when possible.
A second more specific reverse primer 16S2 was also used to avoid amplification of chloroplast homologues [18].
At least one primer was selected to be outside the region selected for dsRNA expression to avoid amplification of RNA deriving from the dsRNA trigger.
Primers were targeted to the exon-exon junction and RNA extracts were treated with DNase (Fermantas International inc, Burlington, ON, Canada) to avoid amplification of contaminant DNA.
The primer pairs were designed to span at least one intron in order to avoid amplification of the contaminating genomic DNA along with cDNA.
All of the primers for Q-RT-PCR were designed to span exon junctions so as to avoid amplification of any potential contaminating genomic DNA.
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