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Phylogenetic analyses excluded gap regions to avoid alignment ambiguities in regions with variation in sequence lengths.
To avoid alignment errors we retained only those with >60% identity at the protein level.
To avoid alignment efficiency biases caused by the presence of the insertion, the allele ratios were subsequently requantified using local realignment with the reference and mutated sequences.
The anchor length of the software was set at 8 nt and the mismatch number in these regions at 0 nt to avoid alignment bias.
Indeed, most WSP and other OMPs-based studies have excluded the HVRs to avoid alignment issues, although this resulted in exclusion of crucial functional domains of the protein.
In order to avoid alignment bias, qAlign will automatically inject the known single nucleotide variations into the reference genome to produce two new versions of that genome.
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But it allowed us to avoid alignments that are obviously suboptimal, and we could show that these class-3 alignments produce stable tree topologies.
This measurement should avoid alignment mistakes between the polarization direction of the incoming light and the polarizer, since minor changes in the value of the TE polarization can significantly effect the extinction ratio.
In order to avoid this alignment problem, we propose an alignment based on an alphabet composed of three letters (ACT or AGT) instead of a four-letter alphabet (ACGT).
The weight factor c1 is an adjustable parameter for balancing the profile term and the secondary structure matches; the shift constant c2 is introduced to avoid the alignment of unrelated regions in the local alignment [ 8].
Only the two most significant alignments were retained in an attempt to avoid spurious alignment to multiple genes from different stickleback linkage groups (for example due to similarity of genes from the same gene family).
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