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BV-2 cells were seeded at a density of 5 × 104 cells in each well of 96-well plates and allowed to attach over night, and then incubated with 1 μg/mL LPS for 6 h with or without 50 μM C60 COOH pretreatment for 6 h.
BB1 cells were seeded at a density of 1×105 cells/100 mm-dish and allowed to attach over 24 h.
Cells were allowed to attach over night and treated in the dark with different drug combinations as indicated in the Results section.
Cells were plated and allowed to attach over night before cells were washed with fresh medium to remove debris.
The cell seeding day was deemed Day 0. Cells were allowed to attach over an initial 3-day period with medium bathing both sides of the filter support.
For these assays, cells were seeded into 96-well plates (PerkinElmer Inc., Wellesley, MA, USA), left to attach over night, before test compounds were added.
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Scaffolds for tissue engineering should be able to attach cells over it, should facilitate cell proliferation and osteoinduction and should be least cytotoxic for its better acceptability.
MSCs were able to attach and spread over the material surface taking advantage of the surface microporosity and topography.
When hairless laboratory mice were restrained within wire mesh tubes and larvae were allowed to attach at random over their bodies, 13.6% of these larvae became infected with B. afzelii if they fed 3 days after the attachment of a single infected nymph (i.e., transmission probability of 0.136, as used below).
Use a staple, washer head nail, or other fastener to attach the FlexWrap over the wall.
For comfort and practicality it is recommended that you wear a top or shirt that buttons up to the neck, as this makes it easier to attach the hood over your gown.
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CEO of Professional Science Editing for Scientists @ prosciediting.com