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Then, they were seeded onto 22 × 22 mm glass coverslip slides and allowed to attach for one hour.
Cells were left to attach for 4 6 hours before treatment.
The cells were placed in a 96-well plate and allowed to attach for 24 h.
SH-SY5Y Cells (2 × 104/well) were seeded in a 96-well plate and allowed to attach for 24 h.
Cells (1 × 105/well) were seeded in 24-well plate and allowed to attach for 24 h.
Briefly, SH-SY5Y cells (2 × 104/well) were seeded into 96-well plates and allowed to attach for 24 h.
Cells were left to attach for 4 6 hours and treated with gold nanoparticles for 24 hours.
SH-SY5Y cells (1 × 106/75 cm2 flask) were seeded and allowed to attach for 24 h.
In brief, the cells (1 × 105/well) were seeded in 24-well plate and allowed to attach for 24 h.
For cytotoxicity assay, 1 × 104 cells were seeded in a 96-well plate and allowed to attach for overnight.
After allowing the cells to attach for 2 4 h, the inserts were washed with phosphate buffered saline (PBS) and then suspended in stirred media to grow.
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