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Reverse genomics consists of identifying a specific sequence, either coding or non-coding, and try to assign a function using sequence comparison or functional analysis.
In silico functional annotation based on protein signatures and homologies allowed us to assign a function to 43% of the 11,839 predicted proteins.
Of these 215 genes, an E > 10−10 was used to assign a function for 205 genes, and a value in the range of 10−7 to 10−10 had to be used to assign a function for about 10 genes.
Thus, further study is required to assign a function to NPG7.
In plants, evidence is lacking to assign a function as signaling molecules to them because plant receptors binding GABA or GHB are still to be identified.
The need to assign a function to newly identified protein sequences has driven research into the conservation of protein functions as sequences diverge.
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The genetic ablation of these loci, while technically challenging, would be key to assigning a function for these elements.
Genes more highly expressed in iL3 stages are, comparatively, less easy to assign a role or function to them.
Because many Ae. aegypti genes have yet to be annotated with specific gene functions we attempted to assign a probable function to them by searching several databases (VectorBase, SwissProt, and NCBI) for similar genes with known functions (Table 7).
The information within the criteria is referred to assign a preference function to each criterion.
In most cases, irony is easily recognisable, but owing to its ambiguity, it is sometimes difficult to assign a specific function to it.
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