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We therefore devised an alternative way to assay the function of the Wnt gpi anchor in Wnt secretion.
There was no test to assay the function of lamin variants in vivo.
Furthermore, addition of ascorbic acid and TMPD to assay the function of complex IV revealed a smaller increased of oxygen consumption in MDA231 cells compared to BT474 cells.
To assay the function of set1/mll family members in planarian regeneration, we fed animals bacterially expressed dsRNA targeting each gene then amputated the animals anterior to the pharynx and observed them during regeneration.
All of the Ig-fold variants tested here caused dominant muscle defects, similar to laminopathies in humans, suggesting that Drosophila can be used to assay the function of novel sequence variants for their pathogenicity.
We used them to assay the function of different regions of talin in three distinct integrin-mediated developmental processes: (1) muscle attachment in the embryo; (2) epidermal morphogenesis during early embryogenesis; and (3) adhesion between the two epithelial cell layers of the adult wing.
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To assay the functions of the sense-oriented genes, we fused V2 V1 to the IR, forming a TYLCV-derived 1,467-bp 1,467-bpn pDRinsertnd named in "pDRIVE.
In addition, these data help to interpret experiments that assay the function of T1 tRNATyr on the ribosome discussed in the next section.
To assay the molecular function of C. elegans SMF-1, -2 and -3 proteins as divalent-metal transporters, we transvected the cDNA encoding each of the C. elegans proteins into the ΔSMF1+2 yeast mutant, and tested these transgenic strains for hypersensitivity to EGTA.
The availability of tools to engineer the C. elegans genome (Golic 2013) might allow this question to be addressed by assaying the function of selected operons compared with their individual component genes each expressed under their own promoters.
Here, we assayed the function of the BLM homolog OsRecQl4 during DNA replication in rice via knockout mutants.
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