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The resulting subclones were grown on CMC agar to assay for cellulase activity.
This test was done in order to assay for cytotoxicity by measuring cell proliferation.
Using knock-out mice we chose to study cataractogenesis in the lens and oncogenic transformation in mouse embryo fibroblasts to assay for effects of ATM deficiency.
The screening strategy incorporates two mesodermal markers, Xbra and Xmyf-5, to assay for cell fate specification and patterning, respectively.
Twenty-five microfitres of cell suspension was transferred to a new 96-well plate and used to assay for protein content.
We used light scattering to assay for assembly.
Untransfected HEK293 cells were included in all trials as negative controls to assay for background noise.
Mice were primed and boosted twice, and serum was collected to assay for antibody titers.
To assay for immobilization, both gross and fine movements were examined.
Mock reactions with no RT were done on each sample to assay for DNA contamination.
To assay for transfection efficiency, transfections were performed with a constant amount of Renilla expression plasmid.
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