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In order to ascertain the implication of these observations we have analysed the expression of cardiomyocyte markers such as gata-4, cardiac Troponin C (cTnC) and α-myosin heavy chain (α-MHC) in f-fEBs and stEBs at the corresponding stage of embryoid body formation (Figure 6A, B).
Transfection of the cells with siRNA, expression vectors and reporter vectors as well as chromatin immunoprecipitation (ChIP) assay were used to ascertain the implication of Ku proteins on ERBB2 expression.
Reverse transcription polymerase chain reaction (RT-PCR), Western blotting, immunoprecipitation, luciferase assays and chromatin immunoprecipitation (ChIP) assays were used to ascertain the implication of ERβ on HIF-1 function.
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In these circumstances it is highly desirable to locate all specimens that have been dated with the original chronology to ascertain the implications of changes.
I want to ascertain the implications of these asterisks, addendums and annotations.
Further researches with larger patient population are necessary to ascertain the implications of LEP and LEPR polymorphisms in obesity.
However, we recognise that this is a convention and further research needs to be conducted to ascertain the implications of different levels of adherence to different kinds of interventions in different contexts.
In the framework of this project genetically modified mouse models are assessed according to 20 pre-defined standard operating procedures (SOPs) that are linked to measurable physical parameters to ascertain the implications of genetic mutations on phenotypes (Mallon et al., 2012).
Finally, stepwise regression was used to ascertain the significance of AASC to the NSMJC.
To ascertain the specific implication of Fyn kinase in modulating PPARγ Ser-84 phosphorylation by Erk1/2, we found that ectopic expression of Fyn in the presence of GHS-R1a in 293 cells caused a strong inhibition of Erk1/2 phosphorylation, which correlated with a time-dependent decrease of PPARγ Ser-84 phosphorylation in response to ghrelin (Fig. 5D).
To ascertain the real implication of UVA exposure in extracellular matrix changes observed with age in human cornea, we have developed a model of photoaging by chronically exposing corneal stroma keratocytes with a precise UVA irradiation protocol.
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