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The paint layers were separated from the limestone support and the rendering layers to which they are applied, but it was not possible to individually separate the paint/coating layers themselves as they were too thin to allow viable samples to be taken.
Animals were transferred to fresh plates every day during the progeny production period, and the original plates were left at 20°C for 24 hours to allow viable eggs to hatch and were then stored at 4°C to be scored at the end of the experiment.
Our challenge was to selectively induce apoptosis in some cells, sparing vicinal macrophages from death in order to allow viable cells to rescue extruded amastigotes.
For this assay, 10 μL of resazurin (final concentration 100 μM) was added to each well and allowed to incubate at 37 °C with shaking at 250 rpm for 1 h to allow viable cells to convert resazurin to resorufin.
As our aim was to determine the growth characteristics of residual tumor cells, the tumor volume irradiated was designed to allow viable tumor cells outside the high-dose region (subcurative radiation).
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Or ovarian tissue can be removed and frozen in hopes that research now being pursued will one day allow viable eggs to be developed in the laboratory.
However, these prognostic factors do not allow viable prediction of the outcome for the individual patient.
Netanyahu's immediate predecessors, Ehud Olmert and Ariel Sharon, both painfully came to the conclusion that Israel could not survive as a democratic and Jewish nation unless it was willing to allow a viable Palestinian state to be established — which in turn would require abandoning settlements.
The cultures were then diluted 1000-fold into Middlebrook 7H9 medium containing no antibiotic and incubated in a roller incubator at 37°C for 5 days to allow the viable cells to grow.
Though the rate of D-xylose utilization by the evolved strain was much too low to allow industrially viable ethanol production from lignocellulosic feedstocks, the work provided a starting point for the development of strains using a bacterial XI that was less inhibited by xylitol than the fungal Piromyces XI.
After drug incubation of 72 h, 20 µl MTT (4 mg/ml) was added to each well and the plate was further incubated for 4 h, allowing viable cells to develop from the yellow-colored MTT into dark-blue formazan crystals.
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CEO of Professional Science Editing for Scientists @ prosciediting.com