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Authors are expected to provide full details of methodology to allow reproducibility under the same research conditions.
Nonetheless, following the accepted consensus and providing enough information to allow reproducibility are accepted as a positive means for overcoming this limitation.
However, to validate new therapeutic strategies in this obstructive model and to allow reproducibility between different laboratories it is necessary to follow standard operating procedures.
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These key features allow reproducibility of pipeline runs, enable ease of use and provide flexibility to update functions within the pipeline.
Additionally, while learning curve studies often define volume thresholds for improvement, few of these studies demonstrate specific technical modifications that allow reproducibility of improved outcomes.
Many basic techniques that are presented, including rtPCR, Western blotting, immunochemistry, really do not have a rigorous standardisation that will allow reproducibility.
For this research Amatulli implemented a scripting procedure using open source codes to allow full reproducibility of the research.
Said and Bellogín [126] stress the importance of having clear guidelines in comparison of recommender systems to allow for reproducibility and comparison of results.
Hyphal extracts contain gliotoxin, an immunosuppressive mycotoxin, which induces apoptosis of antigen-presenting cells and inhibits transcription factor NF-κB [22] [24] As these formulations contain multiple microbial adjuvants (β-glucan, chitin) and inhibitory molecules, standardization of antigen preparations is necessary to allow for reproducibility and transparency in study results.
The same range of illumination values will be used to allow maximum reproducibility to avoid differences in the illumination, since they can produce significant differences in the results of image quantification.
In addition to the expert judgments, the selection and elicitation processes need to be documented, in order to allow for reproducibility and to improve the interpretation of the results.
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