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Insert membranes were isolated following migration and stained with DAPI to allow for quantification of migrated cells (total magnification, 100×).
Hence, to allow for quantification of bleb survival, we deemed a bleb size that measured ≥50% of the size at day-2 as a functioning bleb, that is, positive for bleb survival whereas a bleb size of <50% was considered a failure and non-survival.
fMRI data were collected using the arterial spin labeling technique to allow for quantification of cerebral blood flow.
The sequelae were considered independently of any particular concept to allow for quantification of each individual sequela.
Calibrated [C]-labelled standards (Amersham Biosciences, Uppsala, Sweden) were incorporated in all cassettes to allow for quantification.
To allow for quantification of artifacts, we developed threshold values that allowed for measuring the volumes affected by PHV-induced artifacts.
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The LOD was low enough to allow for the quantification of spore concentrations since spore yields in fermented cultures are often above 108 spores/mL.
Alternative historic land management scenarios were conducted to allow for the quantification and direct comparison of the effects that different land management decisions had on the C budget of the land base over an extended timeframe.
In order to allow for uniform quantification of changes in correlation between units during different temporal intervals across multiple data sets, only the top correlation pairs from the simultaneously recorded neurons of each mouse were used to compute the statistics.
The assay was started by the addition of mitochondria and the change in absorbance at 340 nm (ε = 6.22 mM−1cm−1) due to NADH oxidation in the presence of ubiquinone-1 was recorded for 1minute, after which 5 µg/ml rotenone was added and the absorbance recorded for a further 2 minutes to allow for the quantification of the rotenone-sensitive activity.
Membranes were reprobed for β-actin to normalize for loading and to allow for accurate quantification.
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