At Loyola University, resin blocks were made from formalin-fixed, paraffin-embedded tissue sections from KD patients 1 and 3 after IHC with synthetic antibody, to allow for accurate localization of ICI within bronchi, as previously described [7].
To allow for anatomical localization and inter-subject comparisons, the tracking results for each participant were spatially normalized into a common space.
Nanoscale or microscale entities are functionalized to recognize molecular signatures and provide sensitivity enhancement in either radiological or optical imaging to allow for cancer localization [ 11, 12].
Additionally, to allow for anatomical localization of functional activation, a T1-weighted anatomical image was obtained for each participant (TR = 9 ms, TE = 3.5 ms, FA = 8º, FOV = 232 × 170 mm, matrix = 256 × 256, slice thickness = 1 mm; 170 coronal slices sequentially acquired).
In some experiments, DNA was stained with DAPI to allow for co-localization with centrosome staining.
The wild-type FLHKII (full-length HKII) protein and the deletion mutants were cloned in-frame with the GFP-tag to allow for observation and localization by fluorescence microscopy.
Hepatocyte and hepatic plate-like architecture has been reproduced in diverse culture systems that provide cell attachment sites on two opposing sides of the cells to allow for the appropriate localization of cell adhesion molecules and cytoskeletal components (e.g. collagen sandwich and EHS Matrix overlay) (Dunn et al., 1991; Ezzell et al., 1993; LeCluyse et al., 1996a; Hamilton et al., 2001).
Analysis of gene expression differences in RNA samples extracted from intact tissues does not specifically allow for localization of the increased or decreased altered genes to the glandular and/or stromal compartments in TZ tissues, both of which likely contribute fundamentally to evolution of BPH, nor does it necessarily translate to altered levels of more biologically relevant proteins.
A more complete description of the porcine genome should allow for localization of those SFPs which fall in coding regions and give rise to non-synonymous changes.
For expertise by fingerprint examiners, holistic processing might support the interpretation of individual features in context with their surroundings or allow for localization of target features when doing visual search during a comparison.
