To allow for alignment ambiguity in the case of frameshift variants, 5 bp upstream and downstream of an indel were extracted and compared.
The genomes should be sufficiently close in order (1) to allow for unambiguous identification of orthologous genes; (2) to retain a considerable fraction of the gene order so that orthologous intergenic regions could be identified as regions between pairs of pairwise orthologous genes; and (3) to allow for alignment of orthologous intergenic regions.
To prepare the data for Genebrowser analysis, the read were aligned to the E. grandis genome using the TopHat aligner [ 63] to allow for spliced alignments.
It does not represent the harmonization of laws and regulations, which is not necessary to allow for the alignment of technical requirements and for greater regulatory cooperation.
To allow for smooth alignment, all co-builders work together in the planning phase to rule out unwanted conflicts between work activities.
The two-step retraction method could still be used where there is a need to retract the canines first to allow for the alignment of the incisors; however, according to these findings, the use of an en masse/miniscrew protocol is more effective in anchorage preservation in maximum anchorage cases.
If a surgical patient has minimal crowding, it might not be outside the realm of possibility that the practitioner might choose to procline the upper incisors to allow for the alignment of the teeth before the patient is sent for surgery.
More generally, these experimental and computational methods can be used to study any DNA or RNA based virus with a known sequence to allow for the alignment of the Illumina reads.
Many decisions were delegated to the regions of medical education to allow for optimal alignment to the local context, and to avoid information overload of the HORA project team.
However, the loss of signal due to radiation must be balanced against the need for sufficient contrast in the images to allow for accurate alignment of the individual particles.
