Salient sounds initially result in a large prediction error (when no adequate top-down predictions are present, the prediction error corresponds to the input), and this may require model reconfiguration at higher levels, which may activate 'voluntary' top-down influence, which, in turn, may additionally amplify the impact of the salient sounds due to greater gain.
Higher plants, C. elegans, and fungi additionally amplify silencing via transitivity, a pathway that produces dsRNA and additional siRNAs from the targeted mRNA [6], [7].
For seven patients with mutated CTCs, we could additionally amplify CTC cell pools (2 14 cells).
To ensure that the copy number of nuclear DNA is sufficient for amplifying nuclear Neandertal DNA and to monitor potential nuclear DNA contamination we additionally amplified a control position on the Y-chromosome, which was previously described as ancestral in both specimens and defines the deepest clade in the human Y-chromosome tree (Y2 in [ 24]).
Furthermore, an increase of glycolysis can be additionally amplified by endogenous secretion of catecholamines, due to stress during hypoxia.
The genomic region covering exon 7 of the MDM4 gene was additionally amplified in 140 breast cancer and 200 control samples to allow for a subsequent restriction-enzyme based screening of the D153G mutation using BbsI (New England BioLabs).
The genomic region covering exon 1 of the MDM4 gene was additionally amplified in 133 breast cancer and 136 control samples to allow for a subsequent restriction-enzyme based screening of the c.-103 T/C variant using MboI (New England BioLabs).
Positive samples were additionally amplified by RT-PCR in the open reading frame 1 (ORF1) according to [ 27], and in the junction gene region between ORF1 and ORF2 using the One-Step RT-PCR Kit (Qiagen, Hilden, Germany).
The link with hypercalcaemia is based on the finding that, in particular in combination with vitamin D analogues, the calcification process can be additionally amplified in the vascular system.
This parameter reduces male density and thus mating rates in N2, which additionally amplifies the loss of males within N2 populations.
