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Minor changes were made to adapt this protocol (for 385K arrays) to the 12-plex format.
It is possible to adapt this protocol, or steps from this protocol, to increase yield for SOLiD library preparation (data not shown).
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It will be possible to adapt this new protocol to map the positions of other proteins, which will help to improve our understanding of the ways in which chromatin regulates access to DNA.
Developers often adapt this protocol to mobile applications by embedding OAuth tokens directly into their mobile applications without realizing their credentials are easily compromised through decompilation.
We also highlight how to adapt the protocol to screen for switches responsive to other ligands.
The annealing temperature was changed from 63°C to 62°C to adapt the protocol to our Real-Time PCR kit.
In our experience, adapting this protocol to culture tubes is inefficient and impractical.
Since the basic steps of this protocol have been detailed elsewhere, here we focus on problems encountered when adapting this protocol to different cell types or tissue sources and modifications that have been successfully applied to troubleshoot these problems.
While this approach provided the benefits of homogenization of the starting population, and consistent cell numbers between replicates, we encountered difficulties in adapting this protocol to our needs.
Under the assumption of lower perforation risk, we later adapted this protocol to a graduated approach using balloons with incremental diameters of 30, 35 and 40 mm, respectively, inflated like before on successive days.
We adapted this protocol for real-time PCR using the CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).
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