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Special care must be taken when applying the recommended guidelines to a GUS.
The enhancer sequences were identified and tested in transient and transgenic plants using P1-rr fusedento fused to a GUS reporter gene [ 19, 20].
Transgenic Arabidopsis thaliana plants were constructed in which the genome contained the 5' upstream region of ASN1 from -148 to +120 fused in a transcriptional fusion to the hygromycin phosphotransferase (HPT2) gene and to a GUS gene [ 30].
We chose to use the tomato polygalacturonase 1 (Tand1) and 4 (TApromotersoters ligated to a GUS reporter gene as indicators for cell separation in tomato (Hong et al. 2000).
We demonstrated that the B1S3 fragment was sufficient to confer the stress induction and tissue-specific developmental expression characteristics of the CBL1 gene promoter to a GUS reporter gene.
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While an additive effect has been observed when the first three or nine amino acids of the polyubiquitin coding region are fused to the N-terminus of a transgene and coupled with a ubiquitin promoter [ 19], these enhancing effects were later attributed to the removal of a GUS 5' untranslated leader sequence [ 59].
The AS1-AS2 complex interacts with PRC2 components and the AS1-AS2 binding site from the BP promoter is sufficient to confer Polycomb repression on a GUS transgene.
A GUS staining experiment by razorblade section was used to further investigate the exact location of GUS gene expression.
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