QoS classes may be configured, and each can use a different replication strategy and utility function.
Since pMTL007C-E2 possesses a different replication origin from pSY6catP (repH and repL, respectively), we constructed a repL derivative of pMTL007C-E2, named pMTL007C-E6, in order to allow direct comparison of the two repL-based intron-containing vectors, pMTL007C-E6 and pSY6catP.
The induction of cross-links is lethal to cells because it blocks DNA replication, so cross-resistance to a different type of replication inhibitor, HU, was also tested.
Similarly, Crenarchaeota contain two or three copies of PCNA that have arisen from gene duplication and form a heterotrimeric structure in which each subunit has specific binding functions to different replication proteins (Grabowski and Kelman 2003; Barry and Bell 2006).
On the basis of this, the CDT2 and INTS7 genes provide a unique example considering that the two genes contribute to a different phenotype, namely, DNA replication and transcriptional regulation, respectively, though both phenotypes are characterized as part of the fundamental process of life.
Cultures were transferred from a source cryoculture-MTP with 96 homogeneous cultures with identical biomass concentrations to a target MTP with different replication tools and replication methods.
Moreover, different replication origins are affected to a different extent by the deletion of either SET2 (compare ARS409 and ARS1524 – WT and Δset2 in Fig. S3) or RPD3 (compare ARS603 and ARS1524 – WT and Δrpd3 in Fig. S3).
This suggests that the Ssb and Ssb2 proteins are designed to work with quite different replication or DNA repair protein systems.
To enable a rigorous comparison of different replication profiles and, in particular, to define more rigorously the unique profile of mrc1 deleted cells, we revisited the data we reported previously describing the replication profiles of mutants with an extended S phase [ 19].
For these studies, the results showed some differences between different replication attempts but overall, that variation was relatively small.
